Hi chaps, just going to work so won't be able to reply until late tonight...
I only know this as I backed several horses at a 1000 between hours and moments just before the off, with the intention of attempting to close them out in play, these were selections I thought had a better chance than their price suggested, (although I did not expect them to win).
The bets failed to fire because I had "insufficient funds" I then tried to manually trade out with my manual bot (Fairbot) and this would not accept them either, it used to be possible to do it in the past, but not now, despite a green figure of £2000 on the selection! I had to increase my bank to cover them.
I think this is because Betfair frequently withdraw a runner at the end of a race, this meaning the SP or previously matched odds reduce (considerably if it's a low odds selection) and any lay bets placed in play DO NOT get the reduction factor applied, meaning what appeared to be a nice green figure suddenly becomes a red figure, I have had this red number in the £hundreds

in my opinion because Betfair did not remove a non runner before the off...